全文获取类型
收费全文 | 35736篇 |
免费 | 2462篇 |
国内免费 | 3392篇 |
专业分类
林业 | 2064篇 |
农学 | 3594篇 |
基础科学 | 1104篇 |
7619篇 | |
综合类 | 13883篇 |
农作物 | 3699篇 |
水产渔业 | 1061篇 |
畜牧兽医 | 4342篇 |
园艺 | 1966篇 |
植物保护 | 2258篇 |
出版年
2024年 | 141篇 |
2023年 | 804篇 |
2022年 | 1379篇 |
2021年 | 1505篇 |
2020年 | 1512篇 |
2019年 | 1661篇 |
2018年 | 1471篇 |
2017年 | 1815篇 |
2016年 | 2219篇 |
2015年 | 2084篇 |
2014年 | 2292篇 |
2013年 | 2953篇 |
2012年 | 3036篇 |
2011年 | 2377篇 |
2010年 | 1912篇 |
2009年 | 1903篇 |
2008年 | 1503篇 |
2007年 | 1669篇 |
2006年 | 1582篇 |
2005年 | 1239篇 |
2004年 | 835篇 |
2003年 | 639篇 |
2002年 | 529篇 |
2001年 | 519篇 |
2000年 | 527篇 |
1999年 | 412篇 |
1998年 | 375篇 |
1997年 | 323篇 |
1996年 | 286篇 |
1995年 | 304篇 |
1994年 | 223篇 |
1993年 | 249篇 |
1992年 | 212篇 |
1991年 | 193篇 |
1990年 | 186篇 |
1989年 | 152篇 |
1988年 | 114篇 |
1987年 | 96篇 |
1986年 | 63篇 |
1985年 | 56篇 |
1984年 | 44篇 |
1983年 | 25篇 |
1982年 | 20篇 |
1981年 | 30篇 |
1980年 | 27篇 |
1979年 | 16篇 |
1978年 | 25篇 |
1977年 | 14篇 |
1976年 | 10篇 |
1955年 | 12篇 |
排序方式: 共有10000条查询结果,搜索用时 19 毫秒
991.
992.
993.
在广东发现了可能被番茄斑萎病毒属(Tospovirus)病毒侵染的西瓜,采用ELISA和RT-PCR法对该西瓜病样进行了检测,西瓜病叶粗汁液不与番茄斑萎病毒(Tomato spotted wilt virus,TSWV)和西瓜银斑驳病毒(Watermelon silver mottle virus,WSMoV)的血清发生反应;利用引物J13/UHP通过RT-PCR可以扩增出约1400 bp的基因片段,该片段包括一个840 bp的核衣壳蛋白ORF,其推导的氨基酸序列与已报道的Melon yellow spot virus(MYSV)NP基因氨基酸序列的同源率都为99%,进化树分析表明侵染广东西瓜的病毒(命名为MYSV-GZ)属于Tospovirus的MYSV血清组。 相似文献
994.
叶面积指数测定方法综述 总被引:4,自引:0,他引:4
叶面积指数(LAI)被定义为单位地面面积上叶面面积总和,是极其重要的植被特征,是表征植被冠层结构最基本的参量之一,它控制着植被的许多生物、物理过程,如光合、呼吸、蒸腾、碳循环和降水截获等。因此如何有效的测定叶面积指数将是我们面临的重大课题。本文阐述了直接收获法、调落叶法等叶面积测定方法,同时也将例举这些叶面积测定方法已测定过的物种范围,并就这些方法中所存在的局限性进行说明。 相似文献
995.
996.
997.
Stephen O. Duke Antonio EvidenteMichele Fiore Agnes M. RimandoFranck E. Dayan Maurizio VurroNicole Christiansen Ralf LooserJohannes Hutzler Klaus Grossmann 《Pesticide biochemistry and physiology》2011,100(1):41-50
Ascaulitoxin and its aglycone (2,4,7-triamino-5-hydroxyoctanoic acid, CAS 212268-55-8) are potent phytotoxins produced by Ascochyta caulina, a plant pathogen being developed for biocontrol of weeds. The mode of action of this non-protein amino acid was studied on Lemna paucicostata. Ascaulitoxin is a potent growth inhibitor, with an I50 for growth of less than 1 μM, almost completely inhibiting growth at about 3 μM. Its action is slow, starting with growth inhibition, followed by darker green fronds, and then chlorosis and death. Most amino acids, including non-toxic non-protein amino acids, reversed the effect of the toxin when supplemented in the same medium. Supplemental sucrose slightly increased the activity. d-Amino acids were equally good inhibitors of ascaulitoxin activity, indicating the amino acid effects may not be due to inhibition of amino acid synthesis. Oxaloacetate, the immediate precursor of aspartate, also reversed the activity. LC-MS did not detect interaction of the compound with lysine, an amino acid that strongly reversed the effect of the phytotoxin. Metabolite profiling revealed that the toxin caused distinct changes in amino acids. Reduction in alanine, paralleled by enhanced levels of the branched chain amino acids valine, leucine and isoleucine and nearly unchanged levels of pyruvate, might indicate that the conversion of pyruvate to alanine is affected by ascaulitoxin aglycone. In addition, reduced levels of glutamate/glutamine and aspartate/asparagine might suggest that synthesis and interconversion reactions of these amino group donors are affected. However, neither alanine aminotransferase nor alanine: glyoxylate aminotransferase were inhibited by the toxin in vitro. Our observations might be explained by three hypotheses: (1) the toxin inhibits one or more aminotransferases not examined, (2) ascaulitoxin aglycone affects amino acid transporters, (3) ascaulitoxin aglycone is a protoxin that is converted in vivo to an aminotransferase inhibitor. 相似文献
998.
Nobuo SasakiTomoyuki Matsumoto Yoshinori IkenakaAkio Kazusaka Mayumi Ishizuka Shoichi Fujita 《Pesticide biochemistry and physiology》2011,100(2):135-139
The nitrofuran antibacterial agent furazolidone (FZ) is still used in veterinary medicine in some countries in the Middle and Far East. The present study aimed to show the effect of FZ on the activity of microsomal enzymes that metabolize FZ, and to identify the enzyme that contributes to FZ metabolism in chickens. Wistar rats and White Leghorn chickens were administered FZ once a day for four consecutive days. FZ metabolism was accelerated by FZ administration in chickens, but not in rats. The elevation of FZ metabolism coincided with the induction of NADPH cytochrome P450 reductase (CPR) activity in chickens, but such induction was not observed in rats. FZ metabolizing activities were inhibited in the presence of a CPR inhibitor (diphenylene iodonium chloride) but not by the addition of archetypal cytochrome P450 inhibitors (CO or n-octylamine). The preset study concluded that FZ accelerated its own metabolism in the chicken by induction of the activity of CPR. 相似文献
999.
Stefan Tresch Jennifer SchmotzKlaus Grossmann 《Pesticide biochemistry and physiology》2011,99(1):86-95
The mode of action of endothall, an herbicide which was reported to inhibit plant protein phosphatases 1 (PP1) and 2A (PP2A), was investigated. For initial characterization, a series of bioassays was used for comprehensive physiological profiling of endothall effects which suggested a phytotoxic mode of action similar to mitotic disrupter herbicides. Unlike known microtubule disrupters, endothall did not inhibit soybean tubulin polymerization in vitro. As shown in meristematic corn root tips, endothall distorted the orientation of cell division plane and microtubule spindle structures which led to cell cycle arrest in prometaphase. In tobacco BY-2 cells, malformed spindles together with prometaphase arrest of nuclei and abnormal perinuclear microtubule patterns were detected as early as 4 h of endothall treatment. These effects were also observed after treatment with other protein phosphatase inhibitors, cantharidin and okadaic acid, which phenocopied the mitotic changes described in tonneau1 (ton1) and tonneau2 (ton2) Arabidopsis mutants. These mutants are defective in TONNEAU2 (TON2) protein, a regulatory subunit of PP2A, which governs cell division plane and microtubule orientation. Therefore, PP2A/TON2 phosphatase complex is suggested to be an in planta molecular target of endothall. However, in BY-2 cells, additional effects of endothall, including inhibition of S-phase initiation and DNA synthesis, detected by 5-ethynyl-2′-deoxyuridine (EdU) incorporation, and condensed nuclei arrested in late mitosis were observed which were not reported in Arabidopsiston1 and ton2 mutants. This result indicates that two additional checkpoints in cell cycle were blocked by endothall which are probably not associated with TON2-pathway inhibition. Possibly, inhibition of PP1 and/or other PP2A protein phosphatases are involved in the regulation of these cell cycle phenomena. 相似文献
1000.
Jalal Pourahmad Mir-Jamal HosseiniSoudeh Bakan Mahmoud Ghazi-Khansari 《Pesticide biochemistry and physiology》2011,99(1):105-110
Paraquat is a highly toxic herbicide that is used in most countries without restriction. The cytotoxic action of paraquat is mediated by reactive radicals that are products of its metabolic reduction in cells. It has already been hypothesized that some angiotensin-converting enzyme inhibitors (e.g., captopril and enalapril) could show antioxidant and radical scavenging activity through their structural thiol groups, increasing antioxidant enzymes production or nitric oxide synthesis. In this study the hepatoprotective effect of captopril and enalapril against paraquat induced oxidative stress cytotoxicity was evaluated in isolated rat hepatocyte. Subtoxic concentrations of captopril (0.2 mM) and enalapril (0.2 mM) significantly (p < 0.05) protected the hepatocytes against paraquat (2 mM) induced oxidative stress cytotoxicity markers including: cell lysis, reactive oxygen species (ROS) generation, lipid peroxidation, glutathione depletion, mitochondrial membrane potential decrease, lysosomal membrane oxidative damage and cellular proteolysis. Moreover, we showed that non-thiol enalapril acts as well as thiol containing captopril at inhibiting oxidative stress cytotoxicity markers. Finally, our results support the hypothesis that it is the increase in nitric oxide synthesis and not the presence of the thiol group that accounts for the antioxidant activity of ACE inhibitors. 相似文献